Differential Accessibility in ATAC-seq

Learning outcomes

• to perform GC-aware normalisation of ATAC-seq data using EDASeq

• to detect differentially accessible regions using edgeR

Introduction

In this tutorial we use an R / Bioconductor packages EDAseq and edgeR to perform normalisation and analysis of differential accessibility in ATAC-seq data.

This tutorial contains several mistakes, and will be amended in near future.